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Published on: May 21, 2020
Transcriptomic adaptations in rice suspension cells under sucrose starvation
Huei-Jing Wang1, Ai-Ru Wan, Chia-Mei Hsu
1Institute of Life Science, National Defense Medical Center, Taipei, Taiwan, ROC.
Plant Molecular Biology
|November 23, 2006
Summary
Rice plants under sucrose starvation exhibit significant gene expression changes, including vacuolar development and altered metabolic pathways. Key transcription factors and cis-elements are identified as crucial for adaptation to low sugar conditions.
Area of Science:
- Plant Molecular Biology
- Plant Physiology
- Genomics
Background:
- Sucrose is vital for plant energy and development, and its depletion triggers substantial cellular changes.
- Understanding gene expression in rice (Oryza sativa) under sucrose starvation is crucial for crop science.
Purpose of the Study:
- To investigate time-course gene expression profiles in rice suspension cells during sucrose starvation.
- To identify genes, metabolic pathways, and regulatory elements involved in plant adaptation to low sugar.
Main Methods:
- Genome-wide gene expression profiling using Agilent rice chips (21495 probes).
- Analysis of metabolic pathways and transcription factor activity.
- Promoter assays (particle bombardment, luciferase activity) to validate cis-element function.
Main Results:
- Sucrose starvation induced vacuolar biogenesis with upregulation of specific vacuolar-related genes (e.g., H+-pyrophosphatase, delta-TIP, OsATG3).
- Metabolic pathways showed coordinated down- and upregulation of biosynthesis and degradation enzymes, respectively.
- Upregulation of transcription factors (bZIPs, NACs, WRKY) and identification of cis-elements (e.g., CTTATCC, AC motifs) responsive to sucrose starvation.
Conclusions:
- Rice cells adapt to sucrose starvation through significant transcriptional reprogramming, including vacuolar development and metabolic adjustments.
- Specific transcription factors and cis-regulatory elements play key roles in mediating the plant's response to low sugar availability.
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