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Improving, murine health surveillance programs with help of on-site enzyme-linked immunosorbent assay

Craig Franklin

    Journal of the American Association for Laboratory Animal Science : JAALAS
    |November 23, 2006
    PubMed
    Abstract

    No abstract available in PubMed .

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    Enzyme-Linked Immunosorbent Assay

    In 1971, Peter Perlman and Eva Engvall developed an Enzyme-linked immunosorbent assay (ELISA or EIA). ELISA differs from western blot in that the assays are conducted in microtiter plates or in vivo rather than on an absorbent membrane.
    There are many different types of ELISAs, but they all involve an antibody molecule whose constant region binds an enzyme, leaving the variable region free to bind its specific antigen.  Enzyme-substrate reaction allows the antigen to be visualized or quantified.