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Expression and function of c-kit in hemopoietic progenitor cells.
M Ogawa1, Y Matsuzaki, S Nishikawa
1Department of Pathology, Kumamoto University Medical School, Japan.
The Journal of Experimental Medicine
|July 1, 1991
Summary
The receptor tyrosine kinase c-kit is crucial for mouse bone marrow. Blocking c-kit function with an antibody halts hemopoiesis, demonstrating its essential role in blood cell development.
Area of Science:
- Hematology
- Molecular Biology
- Immunology
Background:
- The c-kit receptor tyrosine kinase plays a role in cellular signaling.
- Its function in adult bone marrow hematopoiesis is not fully understood.
Purpose of the Study:
- To investigate the expression and function of c-kit in adult mouse bone marrow.
- To determine the role of c-kit in constitutive intramarrow hematopoiesis.
Main Methods:
- Utilized monoclonal antibodies (mAbs) against the extracellular domain of murine c-kit.
- Quantified c-kit expression on mouse bone marrow cells.
- Assessed the impact of c-kit+ cell depletion and antibody-mediated c-kit antagonism on hematopoiesis.
Main Results:
- 7.8% of adult mouse bone marrow cells express c-kit, with some co-expressing myeloid markers.
- Depletion of c-kit+ cells eliminated hemopoietic progenitor cells.
- Administration of an antagonistic anti-c-kit antibody (ACK2) led to the disappearance of progenitor cells and mature myeloid/erythroid cells.
Conclusions:
- Most adult bone marrow hemopoietic progenitors express c-kit.
- c-kit is essential for constitutive intramarrow hematopoiesis.
- c-kit is critical for the self-renewal of hemopoietic progenitor cells at various differentiation stages.