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Related Experiment Videos

9S binding protein for androgens and progesterone.

E M Wilson, O A Lea, F S French

    Proceedings of the National Academy of Sciences of the United States of America
    |May 1, 1977
    PubMed
    Summary

    Researchers identified a 9S steroid binding protein in androgen-responsive organs that binds dihydrotestosterone and progesterone. This abundant intracellular protein may play a role in androgen and progesterone compartmentalization.

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    Area of Science:

    • Biochemistry
    • Molecular Biology
    • Endocrinology

    Background:

    • Steroid hormones like androgens and progesterone play crucial roles in various physiological processes.
    • Understanding the intracellular mechanisms of steroid hormone transport and action is essential.

    Purpose of the Study:

    • To identify and characterize a novel steroid binding protein in androgen-responsive tissues.
    • To investigate the binding properties and potential function of this protein.

    Main Methods:

    • Isolation of a 9S steroid binding protein fraction from tissue supernatants using ultracentrifugation.
    • Characterization of binding affinities for [3H]dihydrotestosterone and [3H]progesterone using equilibrium binding constants.
    • Analysis of protein properties including chromatography, sedimentation, pH stability, and response to chemical agents.

    Main Results:

    • A 9S protein (MW ~200,000) was identified in androgen-responsive organs, notably epididymis and testis.
    • The protein binds dihydrotestosterone (Kd ~10^5 M-1) and progesterone (Kd ~10^6 M-1).
    • Its concentration in epididymis is significantly higher than the androgen receptor, with distinct biochemical properties.

    Conclusions:

    • The 9S binding protein is an intracellular, nonsecretory protein with characteristics distinct from the androgen receptor.
    • Its high concentration and low affinity suggest a role in the intracellular accumulation or compartmentalization of androgens and progesterone.

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