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Biosynthesis of a Flavonol from a Flavanone by Establishing a One-pot Bienzymatic Cascade
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Integrated isotachophoretic preconcentration with zone electrophoresis separation on a quartz microchip for UV
Bo Ma1, Xiaomian Zhou, Gang Wang
1Dalian Institute of Chemical Physics, Graduate School of Chinese Academy of Sciences, Dalian, PR China.
Abstract:
A quartz microchip integrated isotachophoretic (ITP) preconcentration with zone electrophoresis (ZE) separation was fabricated using a novel multi-point pressure method featured in normal temperature and lower pressure during bonding process. ITP followed by subsequential ZE of two flavonoids, quercetin and isorhamnetin on the microchip was performed consecutively on the homemade microfluidic workstation with UV detection, resulting in a decreased detectable concentration of 32-fold, compared to the ZE mode only, and their detection limits decreased down to 0.2 microg/mL and 1.2 microg/mL, respectively.
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