A novel methodology for imaging new bone formation around bioceramic bone substitutes
Related Experiment Video
Updated: Jul 18, 2026

Multimodal Approach to Assess Bone Regeneration and Scaffold Performance
Published on: February 13, 2026
Alejandro A Gorustovich1, Matías G Sivak, María B Guglielmotti
1Research Laboratory, National Atomic Energy Commission (CNEA-Regional Noroeste), A4408FTV Salta, Argentina. alegorustov@ciudad.com.ar
Abstract:
In this study, Frost's bulk-staining in combination with confocal laser scanning microscopy (CLSM) was used to image and characterize ground sections of undecalcified rat bone tissue with in situ bioceramic implants. This was addressed by bulk staining specimens in alcohol-soluble basic fuchsin dye. The ground sections were imaged using CLSM in the confocal fluorescence mode. Confocal images revealed that the newly formed bone could be clearly distinguished from bone marrow and cortical bone, as well as from the implant material.

