Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Lipid lateral mobility and membrane phase structure modulation by protein binding.

Martin B Forstner1, Chanel K Yee, Atul N Parikh

  • 1Department of Chemistry, University of California, Berkeley, California 94720, USA.

Journal of the American Chemical Society
|November 23, 2006
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Homogeneous image-based digital immunoassays with high error tolerance.

Npj imaging·2026
Same author

Disordered protein LAT encodes relative levels of signaling pathways in T cell activation.

Science (New York, N.Y.)·2026
Same author

Entropic depletion of macromolecular solutes induces symmetry-breaking surface wrinkling in myelin figures.

Soft matter·2026
Same author

Protocol for Engineered Compositional Asymmetry Within Nanodiscs.

Membranes·2026
Same author

T cell costimulatory and inhibitory signals di4erentially modulate LAT condensate nucleation propensity after TCR ligation.

bioRxiv : the preprint server for biology·2025
Same author

A unifying model of LAT condensates in reconstitution experiments.

bioRxiv : the preprint server for biology·2025

Cholera toxin binding to GM1 lipids in supported membranes significantly alters probe lipid diffusion and membrane phase structure, especially near the gel-fluid transition temperature.

Area of Science:

  • Biophysics
  • Membrane Biophysics
  • Spectroscopy

Background:

  • Lipid lateral diffusion and membrane phase structure are crucial for cell function.
  • Protein-lipid interactions can modulate membrane properties.
  • Supported membrane models are valuable for studying membrane behavior.

Purpose of the Study:

  • To investigate how cholera toxin binding affects lipid lateral diffusion and membrane phase structure.
  • To characterize the influence of protein binding on lipid dynamics in supported membranes.
  • To determine the sensitivity of these changes to protein coverage and temperature.

Main Methods:

  • Utilized fluorescence correlation spectroscopy (FCS) to measure lipid lateral diffusion.
  • Employed infrared absorption spectroscopy to analyze membrane phase structure.

Related Experiment Videos

  • Investigated supported lipid bilayers incorporating GM1 lipids and cholera toxin.

    • Main Results:

    • Cholera toxin binding to GM1 lipids altered long-range lateral diffusion of probe lipids.
    • This diffusion alteration was amplified near the gel-fluid transition temperature (Tm).
    • Protein binding was confirmed to change the fraction of lipid in the gel phase.

    Conclusions:

    • Protein binding to specific lipids can induce significant changes in membrane dynamics and phase behavior.
    • These effects are particularly pronounced near the lipid phase transition temperature.
    • Even low protein densities can substantially impact membrane properties.