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Related Experiment Videos

Solid-like character of virus solutions.

D W Kupke, J W Beams

    Proceedings of the National Academy of Sciences of the United States of America
    |May 1, 1977
    PubMed
    Summary
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    Biochemistry·1994

    Turnip yellow mosaic virus solutions exhibit solid-like properties, with yield stress increasing with concentration. This behavior is dependent on intact viral RNA and is lost upon damage, indicating a specific structural requirement.

    Area of Science:

    • Biophysics
    • Structural Biology
    • Virology

    Background:

    • Turnip yellow mosaic virus (TYMV) is a plant virus with a distinct structure.
    • Understanding the physical properties of viral solutions is crucial for various applications.
    • Previous studies suggested potential solid-like behavior in viral solutions.

    Purpose of the Study:

    • To investigate the solid-like behavior of turnip yellow mosaic virus solutions.
    • To quantify the yield stress required to liquefy these solutions.
    • To determine factors influencing this behavior, such as viral RNA and concentration.

    Main Methods:

    • Utilized a custom-developed torsion-fiber balance for direct yield stress measurement.
    • Extruded viral RNA from TYMV solutions using alkali treatment.

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  • Tested purified viral capsids with and without RNA.

    • Main Results:

    • Observed solid-like behavior in TYMV solutions after RNA extrusion.
    • Yield stress increased with virus concentration and was maximal at room temperature.
    • Damaged virus (e.g., freeze-thaw) or purified capsids showed no solid-like behavior.
    • Solutions transitioned to liquid-like behavior above specific yield stress thresholds.

    Conclusions:

    • The solid-like behavior of TYMV solutions is dependent on the presence of intact viral RNA.
    • Yield stress is a key parameter characterizing the transition from solid-like to liquid-like states.
    • The observed phenomena are intrinsic properties of the virus structure, not artifacts of measurement methods.