Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Development of an algorithm for peak detection in comprehensive two-dimensional chromatography.

Sonja Peters1, Gabriel Vivó-Truyols, Philip J Marriott

  • 1Polymer-Analysis Group, van't Hoff Institute for Molecular Sciences, University of Amsterdam, Nieuwe Achtergracht 166, 1018 WV Amsterdam, The Netherlands.

Journal of Chromatography. A
|November 23, 2006
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Approaches to using retention indices with coupled column pressure tuning in gas chromatography.

Journal of chromatography. A·2026
Same author

Experimental Design-Based Dispersive Liquid-Liquid Microextraction with GC-FID for Determination of Polycyclic Aromatic Hydrocarbons in Surface Water.

ACS omega·2026
Same author

Evaluation of portable atmospheric-pressure solids analysis probe-quadrupole mass spectrometry and UPLC-MS for the rapid screening of illicit substances in human urine.

Journal of chromatography. A·2026
Same author

Insights into Application of Metaverse and Virtual Platforms with Gas Chromatography: Communication, Concept Understanding, Instrumental Training, Optimization Skill Development, and Database Sharing.

Analytical chemistry·2025
Same author

The observation of band shapes for shape-shifting molecule transformations in comprehensive two-dimensional gas chromatography.

Journal of chromatography. A·2025
Same author

Quantitative profiling of fatty acids in tropical oysters (Crassostrea spp.) using dispersive liquid-liquid microextraction and comprehensive two-dimensional gas chromatography.

Food chemistry·2025

This study introduces a new algorithm for detecting and merging peaks in two-dimensional chromatography (2D-Cr). The method enhances compound identification and quantification in complex samples like GCxGC and LCxLC.

Area of Science:

  • Analytical Chemistry
  • Chromatography

Background:

  • Two-dimensional chromatography (2D-Cr) generates complex data requiring advanced peak detection.
  • Accurate identification and merging of peaks from the same compound are crucial for quantification.

Purpose of the Study:

  • To develop and validate a novel algorithm for peak detection and merging in 2D-Cr.
  • To improve the automated analysis and quantification of complex chromatographic data.

Main Methods:

  • The algorithm first applies 1D chromatography peak detection methods.
  • A decision tree analyzes peak features (retention times, regions) to merge peaks from the same compound.
  • User-configurable options and switches allow data-specific adjustments.

Main Results:

Related Experiment Videos

  • The algorithm successfully detected and merged modulated peaks from the same compound in GCxGC air-freshener samples.
  • Recommendations for optimal algorithm settings were provided.
  • A utility for calculating summed peak areas from merged peaks was developed.

Conclusions:

  • The developed algorithm provides an effective solution for peak detection and merging in 2D-Cr.
  • The method is adaptable for both gas chromatography (GCxGC) and liquid chromatography (LCxLC).
  • Automated quantification is facilitated by the integrated peak area summation utility.