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Updated: Jul 18, 2026
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Quantitative Measurement of Intrathecally Synthesized Proteins in Mice
Published on: November 29, 2019
[Reibergram for C3c intrathecal synthesis evaluation].
Alberto Juan Dorta-Contreras1, Elena Noris-García, Bárbara Padilla-Docal
1Laboratorio Central Liquido Cefalorraquideo (LABCEL), Ciudad de La Habana, Cuba. adorta@infomed.sld.cu
Arquivos De Neuro-Psiquiatria
|November 23, 2006
Summary
The Reibergram, a tool for analyzing protein synthesis in cerebrospinal fluid (CSF), is now validated for C3c, a complement factor degradation product. This method helps assess intrathecal C3c synthesis under various blood-brain barrier conditions.
Area of Science:
- Neurochemistry
- Immunology
- Biochemistry
Context:
- The Reibergram is an established tool for evaluating intrathecal protein synthesis.
- Its application has expanded beyond immunoglobulins to other proteins based on molecular flux and cerebrospinal fluid (CSF) flow rate theories.
- C3c, a complement factor C3 degradation product, shares molecular characteristics with IgG.
Purpose:
- To evaluate the intrathecal synthesis of C3c using the Reibergram method.
- To adapt IgG-based Reibergram constants and graphics for C3c analysis.
- To validate the proposed C3c Reibergram under diverse CSF-blood barrier conditions.
Summary:
- C3c, with a molecular weight of 145 KDa, was quantified in serum and CSF of 27 patients and 27 controls using immunodiffusion.
- The study assumed IgG constants and graphic representations to assess intrathecal C3c synthesis.
- The validity of the C3c Reibergram was demonstrated across various CSF blood barrier conditions.
Impact:
- Establishes the Reibergram as a reliable method for assessing intrathecal C3c synthesis.
- Provides a new diagnostic tool for conditions involving C3c.
- Contributes to understanding protein dynamics within the central nervous system.
