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Related Experiment Video

Updated: Jul 18, 2026

Semi-Automated Isolation of the Stromal Vascular Fraction from Murine White Adipose Tissue Using a Tissue Dissociator
06:08

Semi-Automated Isolation of the Stromal Vascular Fraction from Murine White Adipose Tissue Using a Tissue Dissociator

Published on: May 19, 2023

Adipose tissue induction in vivo.

Filip B J L Stillaert1, Phillip Blondeel, Moustapha Hamdi

  • 1Department of Plastic and Reconstructive Surgery, University Hospital Ghent, Belgium.

Advances in Experimental Medicine and Biology
|November 24, 2006
PubMed
Summary

Creating engineered adipose tissue requires blood vessel growth. This study developed a new histioinductive method combining cells, a vascular pedicle, matrix, and chamber, achieving both fat and blood vessel formation in 3D constructs.

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Area of Science:

  • Biomedical Engineering
  • Tissue Engineering
  • Regenerative Medicine

Background:

  • Adipose tissue engineering in vivo necessitates concurrent angiogenesis for stable 3D constructs.
  • Existing histioconductive strategies using biodegradable scaffolds face challenges like delayed angiogenesis and central necrosis.
  • Ischemic necrosis in engineered tissues is a significant hurdle for long-term viability.

Purpose of the Study:

  • To evaluate a histioinductive approach for adipose tissue engineering.
  • To overcome the limitations of delayed angiogenic induction in biodegradable scaffolds.
  • To achieve concomitant adipogenic and angiogenic induction in a 3D construct.

Main Methods:

  • Combining a precursor cell source, vascular pedicle, supportive matrix, and a space-preserving chamber.

More Related Videos

Isolation, Expansion, and Adipogenic Induction of CD34+CD31+ Endothelial Cells from Human Omental and Subcutaneous Adipose Tissue
10:28

Isolation, Expansion, and Adipogenic Induction of CD34+CD31+ Endothelial Cells from Human Omental and Subcutaneous Adipose Tissue

Published on: July 17, 2018

Related Experiment Videos

Last Updated: Jul 18, 2026

Semi-Automated Isolation of the Stromal Vascular Fraction from Murine White Adipose Tissue Using a Tissue Dissociator
06:08

Semi-Automated Isolation of the Stromal Vascular Fraction from Murine White Adipose Tissue Using a Tissue Dissociator

Published on: May 19, 2023

Isolation, Expansion, and Adipogenic Induction of CD34+CD31+ Endothelial Cells from Human Omental and Subcutaneous Adipose Tissue
10:28

Isolation, Expansion, and Adipogenic Induction of CD34+CD31+ Endothelial Cells from Human Omental and Subcutaneous Adipose Tissue

Published on: July 17, 2018

  • Utilizing a histioinductive strategy for adipose tissue development.
  • Constructing three-dimensional adipose tissue models for evaluation.

    • Main Results:

    • Successful concomitant adipogenic and angiogenic induction was observed.
    • The developed histioinductive approach yielded stable 3D adipose tissue constructs.
    • No central cell necrosis was reported in the engineered constructs after 6 weeks.

    Conclusions:

    • The evaluated histioinductive approach effectively engineers adipose tissue in vivo.
    • Combining key components like a vascular pedicle and supportive matrix promotes simultaneous fat and blood vessel formation.
    • This method offers a promising strategy to overcome necrosis and improve the viability of engineered adipose tissue.