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Updated: Jul 18, 2026

Flow Cytometric Analysis of Biomarkers for Detecting Human Sperm Functional Defects
Published on: April 21, 2022
[Characterization of the mRNA profile in ejaculated spermatozoa from healthy fertile men]
Yang-Xing Zhao1, Qiao-Li Li, Zhao-Xia Wang
1School of Medicine, Shanghai Jiao-tong University, Shanghai 200240, China. yangxingzhao2000@yahoo.com
Objective:
To explore the complexity of mRNA in the ejaculated sperm from healthy fertile men.
Methods:
Semen samples were collected from 10 healthy fathers. The swim-up method was adopted to purify the sperm from possible contamination of somatic cells and the spermatozoal total RNA extracted by Trizol was used for SAGE library analysis.
Results:
A totle of 21 052 SAGE raw tags were sequenced from 877 clones and 2 712 unique tags that occurred at least twice in the library were given further analysis. 19.7% of the unique tags had no match in the existing SAGE map, representing novel genes. Molecular function analysis revealed 67% of unique tags related to protein binding or nucleic acid binding categories, 41% to catalytic activity, 13% to message transducer activity, and 10% to transporter, structural and transcription regulator activity, respectively.
Conclusion:
There exists a complex repertoire of mRNAs in the ejaculated spermatozoa from fertile men.
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