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Specific and sensitive diagnosis of syphilis using a real-time PCR for Treponema pallidum

A G Koek1, S M Bruisten, M Dierdorp

  • 1Amsterdam Municipal Health Laboratory and Department of Infectious Diseases, Amsterdam, The Netherlands.

Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases
|November 24, 2006
PubMed

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Real Time RT-PCR

Real-time reverse transcription-polymerase chain reaction, or Real-time RT-PCR, is an analytical tool used to determine the expression level of target genes. The method involves converting mRNA to complementary DNA with the help of an enzyme known as reverse transcriptase, followed by the PCR amplification of the cDNA. These two processes can be performed simultaneously in a single tube or separately as a two-step reaction.
The real-time quantification of the number of amplified products is...