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Published on: October 23, 2011
[Establishment of miniCTTA multiplex set with fluorescence-labeled primers]
Chao Liu1, Hui-pin Wang, Hong-yu Sun
1Institute of Forensic Medicine, Southern Medical University, Guangzhou 510515, China. liuchaogz@21cn.com
Objective:
To establish a miniCTTA multiplex set including short tandem repeat (STR) markers as CSF1PO, TH01, and TPOX and amelogenin gene, whose amplified fragments are shorter than 130 bp.
Methods:
The length of the gene fragments amplified with fluorescence-labeled primers were analyzed by ABI3100 Genetic Analyzer for genotyping of 100 unrelated individuals, 10 genealogies and 30 highly degraded specimens.
Results:
The genotypes derived form miniCTTA were consistent with the results by AmpFLSTR Identifiler kit.
Conclusion:
MiniCTTA multiplex set is useful for personal identification and paternity test, especially applicable for degraded DNA sample.
