[Dynamic changes of plasma VEGF, SDF-1 and peripheral CD34+ cells in patients with acute myocardial infarction]
Zheng-rong Deng1, Chun Yang, Ai-qun Ma
1Department of Cardiology, First Hospital of Xi'an Jiaotong University, Xi'an 710061, China. milanzhu1975@yahoo.com.cn
Insights
In acute myocardial infarction (AMI), vascular endothelial growth factor (VEGF) and stromal cell-derived factor-1 (SDF-1) levels change, mobilizing stem cells to repair heart damage. These factors play a crucial role in the healing process post-AMI.
Area of Science:
- Cardiovascular Biology
- Stem Cell Biology
- Regenerative Medicine
Context:
- Acute myocardial infarction (AMI) is a leading cause of mortality worldwide.
- Understanding the underlying mechanisms of cardiac repair is crucial for developing effective treatments.
- The role of specific growth factors and cell mobilization in AMI recovery is an active area of research.
Purpose:
- To investigate dynamic changes in plasma vascular endothelial growth factor (VEGF) and stromal cell-derived factor-1 (SDF-1) levels.
- To assess peripheral CD34(+) cell counts in patients with AMI.
- To explore the correlation between these factors and their role in myocardial repair after AMI.
Summary:
- Peripheral CD34(+) cells significantly increased by day 7 post-AMI.
- Plasma VEGF levels were elevated in AMI patients, peaking on day 14, while SDF-1 levels transiently decreased initially before returning to normal.
- VEGF levels correlated with CD34(+) cell counts and cardiac enzyme markers (CK-MB, troponin I), suggesting a role in stem cell mobilization.
Impact:
- Findings indicate that stem cells are mobilized to the injured myocardium during AMI.
- Elevated VEGF and altered SDF-1 levels likely contribute to the mobilization and homing of stem cells.
- This study provides insights into the regenerative potential following AMI and suggests therapeutic targets for enhancing cardiac repair.
Objective:
To investigate the changes in plasma levels of vascular endothelial growth factor (VEGF) and stromal cell-derived factor-1 (SDF-1) and in peripheral CD34(+) cells in patients with acute myocardial infarction (AMI), and explore their role in AMI.
Methods:
Enzyme-linked immunoassay (ELISA) was employed for measuring the levels of VEGF and SDF-1 in AMI patients on days 1, 3, 7, 10, and 14 of onset and in normal control subjects. The absolute counts of CD34(+) in the peripheral blood were measured on days 1, 7, and 14 by flow cytometry in AMI patients, with their myocardial enzyme and troponin I detected and electrocardiography (ECG) and echocardiography (UCG) recorded.
Results:
Peripheral CD34(+) cells obviously increased on day 7 after AMI onset (2.35-/+0.72/microl vs 1.48-/+0.49/micro, P<0.05). VEGF levels were significantly higher in AMI patients than in the control subjects, reaching the peak level and on day 14 (197.56-/+39.87 vs 53.79-/+18.12 pg/ml, P<0.01). SDF-1 level obviously decreased on day 1 after AMI onset (1683.12-/+224.79 vs 2178.67-/+265.34 pg/ml, P<0.01), followed by gradually increased to the control level. Obvious correlation was noted between the level of VEGF on day 7 and the peak level of peripheral CD34(+) cells, and the peak plasma VEGF level was obviously associated with the peak serum CK-MB and troponin I levels.
Conclusion:
The stem cells are mobilized into the peripheral blood in the event of AMI. Obviously increased VEGF level following AMI may persist for at least 2 weeks, whereas SDF-1 level undergoes temporary decrement after AMI. The dynamic changes of VEGF and SDF-1 can be related to the mobilization and homing of the stem cells to the injured myocardium.
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