Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

x121: a localized maternal transcript in Xenopus laevis.

M Kloc1, B A Reddy, M Miller

  • 1Department of Molecular Genetics, University of Texas M.D. Anderson Cancer Center, Houston 77030.

Molecular Reproduction and Development
|April 1, 1991
PubMed
Summary

Researchers cloned and characterized a partial cDNA, x121, revealing an RNA localized in Xenopus oocytes. This RNA exhibits an animal-to-vegetal gradient during early development and may function as a transcriptional activator.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Restless legs syndrome in Parkinson's disease: relationship with quality of life and medication.

Bratislavske lekarske listy·2021
Same author

Presynaptic GABAA Receptors Modulate Thalamocortical Inputs in Layer 4 of Rat V1.

Cerebral cortex (New York, N.Y. : 1991)·2018
Same author

Prevalent placement error of deep brain stimulation electrode in movement disorders (technical considerations).

Bratislavske lekarske listy·2017
Same author

Donor and host photoreceptors engage in material transfer following transplantation of post-mitotic photoreceptor precursors.

Nature communications·2016
Same author

Graft-Infiltrating Macrophages Adopt an M2 Phenotype and Are Inhibited by Purinergic Receptor P2X7 Antagonist in Chronic Rejection.

American journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant Surgeons·2016
Same author

Hermes is a localized factor regulating cleavage of vegetal blastomeres in Xenopus laevis.

Developmental biology·2004

Area of Science:

  • Developmental Biology
  • Molecular Biology
  • Xenopus laevis Research

Background:

  • Xenopus oocytes are a key model for studying early embryonic development.
  • Understanding maternal factors that regulate gene expression is crucial for developmental processes.

Purpose of the Study:

  • To clone and characterize a novel RNA molecule, x121, present in Xenopus oocytes.
  • To investigate the spatial and temporal expression patterns of the x121 RNA.
  • To predict the potential function of the protein encoded by x121.

Main Methods:

  • Partial cDNA cloning and sequencing.
  • RNA localization studies in Xenopus oocytes and embryos.
  • Northern blot analysis to determine RNA expression levels.
  • Amino acid composition analysis of the conceptual protein.

Related Experiment Videos

Main Results:

  • A partial cDNA, x121, was successfully cloned and characterized.
  • The corresponding RNA is localized to the animal hemisphere of Xenopus oocytes.
  • Expression shows an animal-to-vegetal gradient during early cleavage stages.
  • x121 RNA levels decrease significantly from fertilization through the gastrula stage.

Conclusions:

  • The x121 RNA is a maternally deposited factor with dynamic expression during early Xenopus development.
  • The predicted protein shares characteristics with known transcriptional activators, suggesting a role in early gene regulation.