Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Smooth muscle caveolae differentially regulate specific agonist induced bladder contractions.

V Cristofaro1, C A Peters, S V Yalla

  • 1Division of Urology, VA Boston Healthcare System, Brigham & Women's Hospital, Boston, Massachusetts 02132, USA.

Neurourology and Urodynamics
|November 24, 2006
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Radiochemotherapy for salivary gland adenoid cystic carcinoma: survival assessment through a retrospective study exploiting real-world data extracted from data warehouse.

ESMO real world data and digital oncology·2026
Same author

Robotic excision and ureteroureterostomy of blind-ending ureteral 'Duplication'.

Journal of pediatric urology·2018
Same author

Report on The Society for Fetal Urology panel discussion on the selection criteria and intervention for fetal bladder outlet obstruction.

Journal of pediatric urology·2017
Same author

Use of the physical environment to support everyday activities for people with dementia: A systematic review.

Dementia (London, England)·2016
Same author

Reoperative robotic pyeloplasty in children.

Journal of pediatric urology·2016
Same author

A meta-review of stress, coping and interventions in dementia and dementia caregiving.

BMC geriatrics·2016

Caveolae regulate bladder smooth muscle contraction by modulating specific G-protein-coupled receptor signaling. Disrupting caveolae altered responses to certain agonists, highlighting their functional role in bladder tissue.

Area of Science:

  • Cell biology
  • Physiology
  • Biochemistry

Background:

  • Caveolae are cholesterol-rich microdomains in cell membranes.
  • They play a role in signaling protein sequestration and integration of extracellular stimuli.
  • Previous research noted bladder caveolae alterations but lacked functional insights.

Purpose of the Study:

  • Investigate the role of caveolae in modulating receptor-mediated signal transduction in the bladder.
  • Determine the presence and localization of caveolin proteins within bladder tissue.

Main Methods:

  • Measured contractile responses in rat bladder tissue before and after caveolae disruption via cholesterol depletion.
  • Used methyl-beta-cyclodextrin to deplete cholesterol and restore caveolae.
  • Employed RT-PCR, immunohistochemistry, and Western blotting to analyze caveolin expression and localization.

Related Experiment Videos

Main Results:

  • Disrupting caveolae attenuated responses to angiotensin II and serotonin but augmented responses to bradykinin and phenylephrine.
  • Cholesterol replenishment restored contractile responses to baseline levels.
  • Carbachol and KCl-induced contractions remained unaffected; ultrastructure confirmed caveolae changes.
  • All three caveolins (caveolin-1, -2, -3) were detected in bladder tissue, with immunoreactivity in smooth muscle cells.

Conclusions:

  • Caveolae play a significant role in regulating selective G-protein-coupled receptor signaling pathways in bladder smooth muscle.
  • These microdomains differentially regulate bladder smooth muscle contraction in a stimulus-dependent manner.
  • The findings support a functional role for caveolae in bladder physiology.