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Different epitopes on the dendritic cell-associated NLDC-145 molecule during ontogeny
1Department of Cell Biology, Free University, Amsterdam, The Netherlands.
Immunobiology
|November 1, 1990
Summary
Monoclonal antibody 6D2 targets a distinct epitope on the NLDC-145 molecule in mice. This epitope emerges at birth on interdigitating cells, unlike the broader antigen expression during gestation.
Area of Science:
- Immunology
- Developmental Biology
Background:
- The NLDC-145 antigen is a marker for dendritic cells in mice.
- Understanding antigen expression during development is crucial for immunology.
Purpose of the Study:
- To characterize a novel monoclonal antibody, 6D2, targeting the NLDC-145 molecule.
- To investigate the differential expression of NLDC-145 epitopes during mouse ontogeny.
Main Methods:
- Production and characterization of monoclonal antibody 6D2.
- Immunohistochemical analysis of NLDC-145 antigen and 6D2 epitope expression during mouse development.
- Comparison of epitope expression in lymphoid organs and thymus.
Main Results:
- Monoclonal antibody 6D2 recognizes a unique epitope on the NLDC-145 molecule.
- The 6D2 epitope appears on interdigitating cells around birth.
- NLDC-145 antigen is detectable earlier in gestation, with no difference in thymic epithelial cells.
Conclusions:
- The 6D2 antibody identifies a specific epitope on the NLDC-145 molecule with distinct temporal expression.
- This suggests differential regulation of NLDC-145 epitopes during dendritic cell development.