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A Microphysiological System to Study Leukocyte-Endothelial Cell Interaction during Inflammation
Published on: December 9, 2021
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Human leukocytes express ephrinB2 which activates microvascular endothelial cells
David O Zamora1, Bobby Babra, Yuzhen Pan
1Department of Ophthalmology, Casey Eye Institute, Oregon Health & Science University, Portland, 3375 S.W. Terwilliger Blvd., Portland, OR 97239-4197, USA. zamorad@ohsu.edu <zamorad@ohsu.edu>
Cellular Immunology
|November 25, 2006
Summary
EphrinB2 signaling in lymphocytes may activate endothelial cells (EC) during inflammation. This interaction could be a novel pathway regulating cell migration and adhesion in inflammatory responses.
Area of Science:
- Cell Biology
- Immunology
- Vascular Biology
Background:
- EphrinB2-EphB4 interactions influence cell migration and adhesion.
- Endothelial cells (EC) and peripheral blood leukocytes (PBLs) are key players in inflammation.
Purpose of the Study:
- To investigate the role of Ephrin/Eph signaling in EC-leukocyte interactions during inflammation.
- To determine if EphrinB2 expressed by PBLs can modulate EC behavior.
Main Methods:
- PBLs were isolated and stimulated to analyze EphrinB2 expression.
- Human iris tissue and iris EC (HIEC) were examined for EphB4 expression.
- HIEC were treated with soluble EphrinB2/Fc to assess signaling pathway activation.
Main Results:
- PBLs showed differential EphrinB2 mRNA and protein expression based on subtype and stimulus.
- Blood vessels in iris tissue expressed EphB4, but HIEC EphB4 expression was not altered by stimulation.
- HIEC treated with sEphrinB2/Fc exhibited changes in phospho-Erk1/2 levels.
Conclusions:
- Stimulated lymphocytes express EphrinB2, suggesting a potential to activate EC.
- This EphrinB2-EphB4 pathway represents a novel communication mechanism between EC and lymphocytes in inflammation.
- Understanding this interaction may reveal new therapeutic targets for inflammatory diseases.
