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Updated: Jul 18, 2026

LDL Cholesterol Uptake Assay Using Live Cell Imaging Analysis with Cell Health Monitoring
Published on: November 17, 2018
Glabrol, an acyl-coenzyme A: cholesterol acyltransferase inhibitor from licorice roots
Jung Ho Choi1, Mun-Chual Rho, Seung Woong Lee
1Natural Medicine Research Center, Korea Research Institute of Bioscience and Biotechnology, 52 Eoun-dong, Yusong-gu, Daejeon 305-333, Republic of Korea.
Abstract:
Acyl-coenzyme A: cholesterol acyltransferase (ACAT) esterifies free cholesterol in the liver and the intestine. It has relations with production of lipoproteins and accumulation of cholesteryl esters of the atheroma. Therefore, ACAT inhibitors may act as antihypercholesterolemic and antiatherosclerotic agents. One isoprenyl flavonoid was isolated from ethanol extract of licorice roots. On the basis of spectral evidences, the compound was identified as glabrol (1). Compound 1 inhibited rat liver microsomal ACAT activity with an IC(50) value of 24.6 microM and decreased cholesteryl ester formation with an IC(50) value of 26.0 microM in HepG2 cells. In addition, 1 showed a non-competitive type of inhibition against ACAT.
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