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Related Experiment Videos

Actin dynamics in growth cones.

S Okabe1, N Hirokawa

  • 1Department of Anatomy and Cell Biology, School of Medicine, University of Tokyo, Japan.

The Journal of Neuroscience : the Official Journal of the Society for Neuroscience
|July 1, 1991
PubMed
Summary
This summary is machine-generated.

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Actin monomers continuously add to the nerve growth cone's leading edge, forming new filaments. These assembled actin filaments then flow backward within the growth cone, driving its movement and development.

Area of Science:

  • Neuroscience
  • Cell Biology
  • Cytoskeletal Dynamics

Background:

  • The nerve growth cone is crucial for neuronal development and guidance.
  • Understanding actin dynamics is key to deciphering growth cone motility.

Purpose of the Study:

  • To elucidate the mechanism of actin incorporation and turnover in nerve growth cones.
  • To investigate the spatial and temporal dynamics of actin filaments within growth cones.

Main Methods:

  • Immunoelectron microscopy of cultured neurons injected with biotin-labeled actin.
  • Low-light-level video microscopy of cultured neurons injected with fluorescently labeled actin and photobleached.

Main Results:

  • Actin incorporation occurs preferentially at the membrane-associated ends of existing filaments in the growth cone periphery.

Related Experiment Videos

  • Photobleaching experiments revealed a rearward translocation of actin filaments from the leading edge towards the base of the growth cone.
  • Evidence suggests a continuous rearward flow of actin polymers within the growth cone.

    • Conclusions:

    • Actin dynamics in nerve growth cones involve continuous monomer addition at the leading edge.
    • Assembled actin filaments undergo rearward translocation, contributing to growth cone advance and neurite outgrowth.