Related Experiment Video

Updated: Jul 18, 2026

Bronchoalveolar Lavage of Murine Lungs to Analyze Inflammatory Cell Infiltration
07:03

Bronchoalveolar Lavage of Murine Lungs to Analyze Inflammatory Cell Infiltration

Published on: May 4, 2017

Elevated soluble ADAM8 in bronchoalveolar lavage fluid in patients with eosinophilic pneumonia

Osamu Matsuno1, Eishi Miyazaki, Shinichi Nureki

  • 1Division of Respiratory Disease, Department of Brain and Nerve Science, Oita University Faculty of Medicine, Yufu City, Oita, Japan. matsuno@med.oita-u.ac.jp

International Archives of Allergy and Immunology
|November 25, 2006
PubMed
Abstract

Insights

ADAM8 is linked to eosinophilic lung inflammation. Elevated ADAM8 levels correlate with specific eosinophilic pneumonia types, suggesting its role in human allergic responses.

Area of Science:

  • Immunology
  • Biochemistry
  • Pulmonology

Background:

  • ADAM (a disintegrin and metalloprotease) proteins are key in proteolysis and cell adhesion.
  • ADAM8, a specific member, is implicated in allergic inflammation and acts as a sheddase for VCAM-1 and CD23.
  • VCAM-1 and CD23 are crucial in immune cell trafficking and allergic responses.

Purpose of the Study:

  • To investigate the role of ADAM8 in the pathogenesis of eosinophilic pneumonia (EP).
  • To quantify soluble ADAM8 (sADAM8) and its substrates (sVCAM-1, sCD23) in different EP subtypes.
  • To establish correlations between sADAM8, sVCAM-1, and sCD23 levels in EP.

Main Methods:

  • Measurement of sADAM8, sVCAM-1, and sCD23 in bronchoalveolar lavage fluid.
  • Analysis of patient samples from acute eosinophilic pneumonia (AEP), chronic idiopathic eosinophilic pneumonia (CEP), and drug-induced eosinophilic pneumonia (drug-EP).
  • Statistical correlation analysis between measured biomarker concentrations.

Main Results:

  • Increased concentrations of sADAM8 and sVCAM-1 were observed in both AEP and CEP.
  • Elevated sCD23 levels were specifically found in AEP.
  • A significant positive correlation between sADAM8 and both sVCAM-1 and sCD23 was identified in AEP, but not in CEP.

Conclusions:

  • The distinct patterns of ADAM8 involvement highlight differing pathogenetic mechanisms across EP subtypes.
  • These findings represent the first evidence linking ADAM8 to human eosinophilic responses and lung inflammation.
  • ADAM8 emerges as a potential biomarker and therapeutic target in specific forms of eosinophilic lung disease.

More Related Videos

Bronchoalveolar Lavage Exosomes in Lipopolysaccharide-induced Septic Lung Injury08:27

Bronchoalveolar Lavage Exosomes in Lipopolysaccharide-induced Septic Lung Injury

Published on: May 21, 2018

Related Concept Videos

Asthma I: Introduction01:28

Asthma I: Introduction

Asthma is a chronic inflammatory disorder of the airways characterized by variable airflow obstruction and heightened bronchial responsiveness to a wide range of triggers. The underlying inflammation leads to airway swelling, mucus hypersecretion, and smooth muscle constriction, all of which narrow the airway lumen and impede airflow. Clinically, asthma presents with recurrent episodes of wheezing, shortness of breath, chest tightness, and coughing, symptoms that typically vary in intensity and...
Atypical Pneumonia01:14

Atypical Pneumonia

Atypical pneumonia, often caused by Mycoplasma pneumoniae, is a form of pulmonary infection that differs from the classical presentation of bacterial pneumonia in both its cause and clinical symptoms. Mycoplasma pneumoniae is a pleomorphic bacterium notable for its lack of a rigid cell wall. This structural characteristic imparts resistance to beta-lactam antibiotics and significantly influences the bacterium’s behavior within the human host.Other pathogens responsible for the disease include...