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Updated: Jul 18, 2026
09:36
RNA Pull-down Procedure to Identify RNA Targets of a Long Non-coding RNA
Published on: April 10, 2018
Summary
Designing specific small interfering RNAs (siRNAs) is crucial for gene silencing. This study presents methods to create siRNAs that can differentiate between closely related gene variants, improving therapeutic precision.
Area of Science:
- Molecular Biology
- Genetics
- Biotechnology
Background:
- Small interfering RNAs (siRNAs) are key tools for gene silencing.
- Distinguishing between similar gene variants is a challenge in siRNA design.
- Off-target effects can limit siRNA therapeutic applications.
Purpose of the Study:
- To develop a method for designing highly specific siRNAs.
- To enable siRNAs to discriminate between closely related gene variants.
- To enhance the precision of gene silencing therapies.
Main Methods:
- Computational analysis of siRNA-target interactions.
- In silico prediction of variant discrimination.
- Experimental validation of designed siRNAs.
Main Results:
- Successfully designed siRNAs with high specificity for target variants.
- Demonstrated ability of siRNAs to distinguish between single nucleotide polymorphisms (SNPs).
- Validated computational models for predicting siRNA specificity.
Conclusions:
- The developed method allows for precise siRNA design.
- This approach can minimize off-target effects in gene silencing.
- Enhances the potential of siRNA-based therapeutics.
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Small interfering RNAs, or siRNAs, are short regulatory RNA molecules that can silence genes post-transcriptionally, as well as the transcriptional level in some cases. siRNAs are important for protecting cells against viral infections and silencing transposable genetic elements.
In the cytoplasm, siRNA is processed from a double-stranded RNA, which comes from either endogenous DNA transcription or exogenous sources like a virus. This double-stranded RNA is then cleaved by the ATP-dependent...
In the cytoplasm, siRNA is processed from a double-stranded RNA, which comes from either endogenous DNA transcription or exogenous sources like a virus. This double-stranded RNA is then cleaved by the ATP-dependent...
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Small interfering RNAs, or siRNAs, are short regulatory RNA molecules that can silence genes post-transcriptionally, as well as the transcriptional level in some cases. siRNAs are important for protecting cells against viral infections and silencing transposable genetic elements.
In the cytoplasm, siRNA is processed from a double-stranded RNA, which comes from either endogenous DNA transcription or exogenous sources like a virus. This double-stranded RNA is then cleaved by the ATP-dependent...
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This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...
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RNA interference (RNAi) is a process in which a small non-coding RNA molecule blocks the post-transcriptional expression of a gene by binding to its messenger RNA (mRNA) and preventing the protein from being translated.
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02:47Ribozymes
The term ribozyme is used for RNA that can act as an enzyme. Ribozymes are mainly found in selected viruses, bacteria, plant organelles, and lower eukaryotes. Ribozymes were first discovered in 1982 when Tom Cech’s laboratory observed Group I introns acting as enzymes. This was shortly followed by the discovery of another ribozyme, Ribonulcease P, by Sid Altman’s laboratory. Both Cech and Altman received the Nobel Prize in chemistry in 1989 for their work on ribozymes.
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