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Published on: April 23, 2019
Quality control Fourier transform infrared determination of diazepam in pharmaceuticals
Javier Moros1, Salvador Garrigues, Miguel de la Guardia
1Department of Analytical Chemistry, University of Valencia, 50 Dr. Moliner Street, 46100 Burjassot, Valencia, Spain.
Abstract:
A quality control procedure has been developed for the determination of diazepam in pharmaceuticals using Fourier transform infrared (FTIR) spectroscopy. The method involves the off-line extraction of diazepam with chloroform by sonication and direct determination in the extracts through peak area measurement in the interval between 1672 and 1682 cm(-1) using a baseline correction defined between 1850 and 1524 cm(-1). For standardization it was used an external calibration line established from standard solutions of diazepam in chloroform. The method provides a limit of detection of 0.04 mg per tablet (n=5), a relative standard deviation (R.S.D.) of 0.5% for 5 independent measurements of a standard solution at a concentration level of 0.76 mg g(-1) and a sampling frequency of the whole procedure of 4 h(-1), being required only 45 s for the measurement step. Results obtained by FTIR agree with those obtained by a reference methodology based on ultraviolet spectrometry and thus the developed procedure offers a good alternative for the determination of diazepam in pharmaceuticals.
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