Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Search research articles

Related Experiment Video

Updated: Jul 18, 2026

Generation of Two-color Antigen Microarrays for the Simultaneous Detection of IgG and IgM Autoantibodies

Generation of Two-color Antigen Microarrays for the Simultaneous Detection of IgG and IgM Autoantibodies

Published on: September 15, 2016

Antibody arrays for quantitative immunophenotyping.

Koichi Kato¹, Mitsuaki Toda, Hiroo Iwata

¹Institute for Frontier Medical Sciences, Kyoto University, 53 Kawahara-cho, Shogoin, Sakyo-ku, Kyoto 606-8507, Japan.

|November 28, 2006

Summary

This study presents a new antibody array for detecting multiple cell surface antigens on living cells. This method enables rapid cell phenotyping and quantitative assessment, advancing cell processing and clinical diagnostics.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Adhesion-triggered rapid recruitment/exclusion of membrane-tethered ligands at cell-cell interface.

Nanoscale·2026

Same author

Acceptance of Murine Islet Allografts Without Immunosuppression in Inguinal Subcutaneous White Adipose Tissue Pretreated With bFGF.

Diabetes·2022

Same author

Dual-phase Au-Pt alloys free from magnetic susceptibility artifacts in magnetic resonance imaging.

Magnetic resonance imaging·2021

Same author

Induction of <i>in Vivo</i> Ectopic Hematopoiesis by a Three-Dimensional Structured Extracellular Matrix Derived from Decellularized Cancellous Bone.

ACS biomaterials science & engineering·2021

Same author

Immobilization of Sertoli cells on islets of Langerhans.

Biomaterials science·2020

Same author

A Novel Human Placental Barrier Model Based on Trophoblast Stem Cells Derived from Human Induced Pluripotent Stem Cells.

Tissue engineering. Part A·2020

Area of Science:

Biotechnology
Cell Biology
Immunology

Background:

Accurate detection of multiple surface antigens on living cells is crucial for cell processing and clinical diagnosis.
Existing methods may not offer parallel detection or efficient phenotyping of diverse cell types.

Purpose of the Study:

To develop and validate an antibody array for the parallel detection of multiple cell surface antigens on living cells.
To demonstrate the utility of this array for rapid cell phenotyping and quantitative assessment.

Main Methods:

Fabrication of an antibody array using photo-assisted patterning of an alkanethiol monolayer on a gold-coated glass plate.
Immobilization of antibodies specific for cell surface antigens in an array format.
Direct cell binding assays for phenotyping adhesion-dependent and non-dependent cells.

More Related Videos

Assessment of Resistance to Tyrosine Kinase Inhibitors by an Interrogation of Signal Transduction Pathways by Antibody Arrays

Assessment of Resistance to Tyrosine Kinase Inhibitors by an Interrogation of Signal Transduction Pathways by Antibody Arrays

Published on: September 19, 2018

Related Experiment Videos

Last Updated: Jul 18, 2026

Generation of Two-color Antigen Microarrays for the Simultaneous Detection of IgG and IgM Autoantibodies

Generation of Two-color Antigen Microarrays for the Simultaneous Detection of IgG and IgM Autoantibodies

Published on: September 15, 2016

Assessment of Resistance to Tyrosine Kinase Inhibitors by an Interrogation of Signal Transduction Pathways by Antibody Arrays

Assessment of Resistance to Tyrosine Kinase Inhibitors by an Interrogation of Signal Transduction Pathways by Antibody Arrays

Published on: September 19, 2018

Main Results:

The antibody array allows parallel detection of multiple surface antigens on living cells.
Rapid cell phenotyping was successfully demonstrated for both adhesion-dependent and non-dependent cells.
The density of bound cells on antibody spots correlated with their concentration in the original suspension, indicating quantitative potential.

Conclusions:

The developed antibody array is a feasible method for quantitative assessment of multiple antigen expression on living cells.
This technology has the potential to expand applications in fundamental research and clinical diagnostics.
The array facilitates efficient and parallel analysis of cell surface markers.