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Multiple integrins share the ability to induce elevation of intracellular pH
M A Schwartz1, D E Ingber, M Lawrence
1Department of Cellular and Molecular Physiology, Harvard Medical School, Boston, Massachusetts.
Experimental Cell Research
|August 1, 1991
Summary
Cell adhesion via integrins specifically elevates intracellular pH (pHi) across various cell types. This pH rise is a conserved property of many integrin family members, impacting cellular functions.
Area of Science:
- Cell Biology
- Biochemistry
- Immunology
Background:
- Integrin alpha 5 beta 1 binding to fibronectin activates the Na-H antiporter, increasing intracellular pH (pHi).
- This study investigates whether other integrins also influence pHi.
Purpose of the Study:
- To determine if cell adhesion to various ligands via different integrins elevates intracellular pH (pHi).
- To establish if this pH elevation is specific to integrin-mediated adhesion.
Main Methods:
- Assessing intracellular pH (pHi) changes in bovine capillary endothelial cells (BCE) and human T lymphocytes upon adhesion to different extracellular matrix proteins and antibodies.
- Utilizing antibodies against specific integrins (alpha v beta 3, LFA-1) and other cell surface receptors (CD2, ICAM-2, T cell receptor).
Main Results:
- Adhesion to fibrinogen, collagens, laminin, and vitronectin significantly elevated pHi in BCE cells.
- Non-integrin binding ligands (bFGF, concanavalin A, thrombin) did not increase pHi.
- Antibody-mediated adhesion to integrins (alpha v beta 3, LFA-1) also elevated pHi, while antibodies to CD2 or ICAM-2 had minimal effects.
Conclusions:
- Integrin-mediated cell adhesion specifically elevates intracellular pH (pHi).
- This phenomenon is a shared characteristic among diverse members of the integrin family.
- The findings highlight a conserved signaling mechanism initiated by integrin engagement.