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Updated: Jul 18, 2026
11:57
Studying Protein Function and the Role of Altered Protein Expression by Antibody Interference and Three-dimensional Reconstructions
Published on: April 21, 2016
Protein i: interference at protein level by intrabodies
Jayapal Manikandan1, Peter N Pushparaj, Alirio J Melendez
1Department of Physiology, Yong Loo Lin School of Medicine, National University of Singapore, Singapore.
Frontiers in Bioscience : a Journal and Virtual Library
|November 28, 2006
Summary
Intrabodies, intracellular antibodies, offer a powerful alternative to traditional gene modulation techniques for studying protein function. These engineered antibodies can target specific proteins within cells, aiding in drug discovery and functional genomics research.
Area of Science:
- Biotechnology
- Molecular Biology
- Immunology
Background:
- Immunoglobulin molecules are established in vitro tools for protein analysis.
- Targeting intracellular proteins requires specialized approaches beyond conventional methods.
- Existing techniques like gene knockout and RNA interference have limitations in protein function modulation.
Purpose of the Study:
- To review the concept, construction, and applications of intrabodies.
- To highlight intrabodies as a versatile tool for manipulating biological processes.
- To discuss the potential of intrabodies in target identification and validation.
Main Methods:
- Expression of engineered immunoglobulin molecules intracellularly.
- Targeting specific sub-cellular compartments with antibody molecules.
- Review of existing literature on intrabody development and use.
Main Results:
- Intrabodies provide a powerful alternative to modulate protein function and analyze effects.
- They can be designed to target specific domains of proteins.
- Intrabodies show promise for target identification and validation in functional genomics and proteomics.
Conclusions:
- Intrabodies represent a novel and versatile tool for biomedical research.
- They offer advantages over traditional gene modulation techniques.
- While clinical applications may be distant, intrabodies are valuable for early-stage research and drug target validation.
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