Related Experiment Video
Updated: Jul 18, 2026

Measurement of Cyclic Guanosine Monophosphate (cGMP) in Solid Tissues using Competitive Enzyme-Linked Immunosorbent Assay (ELISA)
Published on: July 3, 2025
Inhibitory effect of Panax notoginseng on nitric oxide synthase, cyclo-oxygenase-2 and neutrophil functions
Un-Ho Jin1, Soon-Gi Park, Seok-Jong Suh
1Department of Biological Sciences, Sungkyunkwan University, Chunchun-Dong, Suwon City, Kyunggi-Do 440-746, Korea.
Abstract:
A water extract of Panax notoginseng Buck F.H. Chen. (Arialiaceae) root (PN) is being used as a therapeutic agent to stop haemorrhages and as a tonic to promote health in Korean and Chinese medicine. The pharmacokinetic profiles of PN have not been accurately investigated. The preliminary aim was to elucidate the pharmacokinetic features of PN. First, the prevention of neutrophil functions was assessed. PN inhibited neutrophil functions, including degranulation, superoxide generation and leukotriene B4 production, without any effect on 5-lipoxygenase activity. PN reduced nitric oxide (NO) and prostaglandin (PG)E2 production in mouse peritoneal macrophages stimulated with lipopolysaccharide (LPS) while no influence on the activity of inducible NO synthase (iNOS), cyclo-oxygenase-2 (COX-2) or cyclo-oxygenase-1 (COX-1) was observed. PN significantly reduced mouse paw oedema induced by carrageenan. The results indicate that PN exerts antiinflammatory effects related to the inhibition of neutrophil functions and NO and PGE2 production, which could be due to a decreased expression of iNOS and COX-2.
Related Concept Videos
Nitric Oxide Signaling Pathway
Antianginal Drugs: Nitrates and β-Blockers
Organic nitrates, such as nitroglycerin, play a pivotal role. Once metabolized, they liberate nitric oxide, a molecular marvel. Nitric oxide triggers guanylyl cyclase and augments cGMP production. This biochemical cascade orchestrates the relaxation of vascular smooth muscles, ushering in vasodilation and enhancing coronary blood flow. Administered...
Antihypertensive Drugs: Vasodilators
GPCRs Regulate Adenylyl Cylase Activity
Two...
Antihypertensive Drugs: Angiotensin-Converting Enzyme Inhibitors
Treatment for Pulmonary Arterial Hypertension: Phosphodiesterase Inhibitors
Among the PDE5 inhibitors, sildenafil (Revatio) stands out as a competitive and selective inhibitor. It operates by elevating cellular levels of cGMP and augmenting signaling through the cGMP-PKG pathway, promoting vasodilation. Upon oral...

