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Characterization of a late gene, ORF60 from Bombyx mori nucleopolyhedrovirus

Meng-Fang Du1, Xin-Ming Yin, Zhong-Jian Guo

  • 1College of Animal Science, Zhejiang University, Hangzhou, 310029, China.

Journal of Biochemistry and Molecular Biology
|November 30, 2006
PubMed

Insights

Bombyx mori nucleopolyhedrovirus open reading frame 60 (Bm60) is a late gene encoding a 31 kDa protein. This protein is expressed in the cytoplasm and nucleus during the later stages of BmNPV infection.

Area of Science:

  • Virology
  • Molecular Biology
  • Insect Pathology

Background:

  • Bombyx mori nucleopolyhedrovirus (BmNPV) is a significant pathogen affecting silkworm production.
  • Understanding viral gene expression is crucial for developing effective control strategies.

Purpose of the Study:

  • To characterize the Bombyx mori nucleopolyhedrovirus open reading frame 60 (Bm60).
  • To determine the expression pattern and localization of the Bm60 protein during viral infection.

Main Methods:

  • Reverse transcription polymerase chain reaction (RT-PCR) to detect Bm60 transcripts.
  • Western blot analysis using a polyclonal antibody against Bm60 to detect the protein.
  • Immunofluorescence microscopy to determine the subcellular localization of Bm60.

Main Results:

  • Bm60 transcript and protein expression were detected between 18-72 hours post-infection (p.i.) in BmN cells.
  • Bm60 expression was inhibited by Ara-c, indicating it is a late viral gene.
  • The Bm60 protein, with a molecular weight of 31 kDa, was localized to the cytoplasm and nucleus during infection.

Conclusions:

  • Bm60 is a functional open reading frame (ORF) in the BmNPV genome.
  • The Bm60 protein is expressed late in the viral infection cycle.
  • Bm60 plays a role in the BmNPV infection process.

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