H-DBAS: alternative splicing database of completely sequenced and manually annotated full-length cDNAs based on...

Area of Science:

• Genomics
• Molecular Biology
• Bioinformatics

Background:

• Alternative splicing (AS) is a key mechanism for increasing protein diversity in eukaryotes.
• Understanding AS is crucial for comprehending gene regulation and cellular function.
• Existing databases may lack comprehensive annotations or specific analysis tools for AS variants.

Purpose of the Study:

• To establish a specialized database, H-DBAS, for alternatively spliced human transcripts.
• To provide detailed annotations and analysis capabilities for AS variants.
• To facilitate the study of complex AS patterns and their functional consequences.

Main Methods:

• Curated a collection of 38,664 representative alternative splicing variants (RASVs) from 11,744 human gene loci.
• Annotated AS variants based on manual curation of full-length cDNAs from the H-Invitational annotation meeting.
• Developed the AS Viewer tool for analyzing AS events in the context of full-length cDNAs.

Main Results:

• H-DBAS contains 38,664 RASVs across 11,744 loci.
• Data is retrievable by AS patterns, amino acid/protein motif alterations, GO terms, localization signals, and transmembrane domains.
• The database includes complex AS patterns involving multiple coding sequences (CDS) from a single locus.

Conclusions:

• H-DBAS serves as a valuable, specialized resource for human alternative splicing research.
• The database enables detailed analysis of AS events and their functional implications.
• It supports empirical understanding of the relationship between AS and protein sequence/function.