[Naturally occurring aliphatic polyamines-induced histamine release from rat peritoneal mast cells]

Rat peritoneal mast cells obtained by Percoll gradient were incubated with different concentrations of naturally occurring aliphatic polyamines, spermine and spermidine, at 0.1-10 mM and the amount of histamine release into the supernatant solutions was measured. The addition of each polyamine to the suspensions of the mast cells caused a histamine release in a dose-dependent manner. The histamine release from the cells incubated with each polyamine was rapid and the amount of the histamine release into the supernatant solutions reached maximum at one minute with the incubations.