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Genetic Screens

Genetic Screens

Genetic screens are tools used to identify genes and mutations responsible for phenotypes of interest. Genetic screens help identify individuals or a group of people at risk of developing genetic diseases and help them with early intervention, targeted therapy, and reproductive options.
Forward genetic screens
Forward or “classical” genetic screens involve creating random mutations in an organism’s DNA using radiation, mutagens, or insertion of additional bases, which result in visible changes...

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Related Experiment Video

Updated: Jul 18, 2026

Immunostaining to Visualize Murine Enteric Nervous System Development

Immunostaining to Visualize Murine Enteric Nervous System Development

Published on: April 29, 2015

Genetic screen for mutations affecting development and function of the enteric nervous system.

Julie Kuhlman¹, Judith S Eisen

¹Institute of Neuroscience, University of Oregon, Eugene, Oregon 97403, USA.

Developmental Dynamics : an Official Publication of the American Association of Anatomists

|November 30, 2006

Summary

Researchers screened zebrafish to understand enteric nervous system development and identified mutations affecting neuron number. A correlation was found between enteric neuron loss and gastrointestinal motility defects, providing tools for future studies.

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Area of Science:

Developmental biology
Neuroscience
Genetics

Background:

The enteric nervous system (ENS) is crucial for gastrointestinal function.
Defects in ENS innervation are linked to human diseases.
The genetic underpinnings of ENS development remain largely unknown.

Purpose of the Study:

To identify genes regulating ENS development using a zebrafish screen.
To investigate the relationship between ENS neuron number and gastrointestinal motility.

Main Methods:

Conducted a forward genetic screen in mutagenized zebrafish.
Assessed the number and distribution of enteric neurons.
Developed and utilized a zebrafish gastrointestinal motility assay.
Characterized mutations affecting ENS development and other neural crest derivatives.

Main Results:

Identified novel mutations specifically impacting enteric neurons.
Discovered mutations with broader effects on neural crest derivatives.
Demonstrated a significant correlation between the degree of enteric neuron loss and the severity of motility defects.
Established a functional link between ENS integrity and gastrointestinal function in zebrafish.

Conclusions:

The zebrafish screen successfully identified genetic factors influencing ENS development.
Enteric neuron number is directly correlated with gastrointestinal motility.
The identified mutations and assays provide valuable resources for future research into ENS disorders.