Cloning, expression and identification of the gene of human single-chain variable fragment antibody against Hepatitis

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Y Z Lun1, J Cheng, Y W Zhong

  • 1Department of Biochemistry and Molecular Biology, Dalian Medical University, Dalian 110027, P.R. China.

Acta Virologica
|November 30, 2006
PubMed

Expression of single-chain variable fragment (scFv) antibodies on the surface of bacteriophage is widely used to prepare antibodies with pre-defined specificities. A phage antibody library containing the gene for scFv antibody against Hepatitis B virus surface antigen (HBsAg) was panned with HBsAg immobilized on microtiter plate wells. After five rounds of panning 30 phage clones specific to HBsAg were obtained and one selected clone was sequenced. It was found to consist of 789 bp and its amino acid sequence and specifically detected the respective antigen in the patients but not in healthy persons.

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