Quantitative phase imaging with scanning holographic microscopy: an experimental assessment

Area of Science:

• Optics and Photonics
• Biomedical Imaging
• Microscopy

Background:

• Scanning holography offers versatile imaging modes (coherent and incoherent).
• Quantitative phase information is crucial for detailed biological specimen analysis.

Purpose of the Study:

• To demonstrate the experimental acquisition of quantitative phase information using scanning holographic microscopy.
• To explore the potential of multimodal imaging by combining phase contrast with other contrasts.

Main Methods:

• Utilizing different detector geometries in scanning holography.
• Employing a point detector for coherent holograms to extract complex amplitude and phase.
• Simultaneously capturing incoherent holograms for intensity-based contrasts.

Main Results:

• Successfully obtained quantitative phase information from scanning holography for the first time.
• Demonstrated the capability to capture simultaneous holograms for multimodal 3D reconstructions.
• Integrated quantitative phase contrast with absorption, reflectance, and fluorescence contrasts.

Conclusions:

• Scanning holographic microscopy can now provide quantitative phase contrast alongside other imaging modalities.
• This breakthrough facilitates advanced 3D reconstructions and multimodal analysis of biological specimens.
• Opens new possibilities for in-depth biological studies using advanced imaging techniques.