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Isolation and identification of alpha-amylase producing Bacillus sp. from dhal industry waste
S Thippeswamy1, K Girigowda, V H Mulimani
1Department of Studies in Biochemistry, Gulbarga University, Gulbarga 585 106, Karnataka, India.
Indian Journal of Biochemistry & Biophysics
|December 1, 2006
Abstract:
A bacterial strain was isolated from dhal industry red gram waste and identified as Bacillus. A thermostable extracellular amylase was partially purified from the strain. Optimum temperature and pH for the enzyme were found to be 60 degrees C and 6.5, respectively. The maximum amylase production was achieved with maltose as carbon source. Among the nitrogen sources, peptone and yeast extract produced maximum amylase.
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