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Updated: Jul 18, 2026
A Protocol for Analyzing Hepatitis C Virus Replication
Published on: June 26, 2014
Lyophilized standards for the calibration of real time PCR assay for hepatitis C virus RNA
Lu-nan Wang1, Jian-min Wu, Wei Deng
1Clinical Laboratory, Union Hospital, Tongji Medical College of Huazhong University of Science & Technology, Wuhan 430022, China.
Background:
Since October 1997, an international standard for hepatitis C virus (HCV) nucleic acid amplification technology assay, 96/790, has been available. We compared a series of lyophilized standards with known HCV RNA concentrations against the international standard in fluorescence quantitative PCR detection.
Methods:
A series of lyophilized sera were calibrated by ROCHE COBAS AMPLICOR HCV Monitor test against the international standard and sent to various manufacturers to analyse the samples using their own kits. Then calibration curves from the series were compared with that obtained from the external standard calibration curve with the manufacture's series.
Results:
The standard calibration curve with the series of lyophilized serum showed an excellent correlation (R(2) > 0.98), slope and intercept that were similar to those from the manufacture's series. When the standard calibration curve from the series of lyophilized standards were used to define the values of the given sample, lower coefficients of variation between kits from different manufactures were obtained.
Conclusion:
The results showed that the lyophilized standards could be used to setup the standard calibration curve for clinical HCV RNA quantitative PCR detection.
