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MIP 28 forms channels in planar lipid bilayers.

E Modesto¹, L Barcellos, A C Campos-de-Carvalho

¹Instituto de Biofisica Carlos Chagas Filho, Universidade Federal do Rio de Janeiro, Brasil.

Brazilian Journal of Medical and Biological Research = Revista Brasileira De Pesquisas Medicas E Biologicas

|January 1, 1990

Summary

Chicken lens main intrinsic protein (MIP 28) forms channels in lipid bilayers, similar to bovine MIP 26. These channels exhibit voltage dependence and their properties are unaffected by detergent treatment.

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Area of Science:

Ophthalmology
Biophysics
Membrane Biology

Background:

Main intrinsic protein (MIP) is abundant in lens fiber cell membranes.
MIP exhibits species-specific variations, such as MIP 26 (bovine) and MIP 28 (chicken).
Previous studies suggest MIP 26 forms channels in lipid bilayers.

Purpose of the Study:

To isolate and characterize channel activity of chicken lens MIP 28.
To investigate the effect of detergent treatment on MIP 28 channel properties.
To compare MIP 28 channel characteristics with those of MIP 26.

Main Methods:

Isolation of membrane fractions enriched in chicken MIP 28.
Incorporation of membrane fractions into planar lipid bilayers.
Electrophysiological recordings of channel activity.

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Functional assays using antibodies and detergent treatment.

Main Results:

MIP 28-enriched fractions exhibited channel activity in lipid bilayers.
Detergent treatment (Triton X-100) did not alter channel properties.
Antibody blockade attempts against MIP were unsuccessful.
Single channel conductance was measured (300-400 pS in 300 mM K2SO4).
Channels displayed voltage dependence and qualitative similarity to MIP 26 channels.

Conclusions:

Chicken lens MIP 28 forms functional ion channels in lipid bilayers.
MIP 28 channel properties are robust to detergent treatment.
The incorporation method suggests MIP may not span the entire bilayer.
Further research is needed to elucidate the precise structure and function of MIP channels.