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Development of an immunosensor for the diagnosis of bovine anaplasmosis

Marta Silva1, Silvina Wilkowsky, Susana Torioni De Echaide

  • 1Instituto de Biologia Experimental e Tecnológica/Instituto de Tecnologia Química e Biológica, Apartado 12, P-2781-901 Oeiras, Portugal.

Annals of the New York Academy of Sciences
|December 1, 2006
PubMed

Insights

An optical immunosensor detects Anaplasma marginale antibodies in cattle using recombinant major surface protein 5 (MSP5). This tool aids in identifying infection and vaccination status in cattle sera.

Area of Science:

  • Veterinary Immunology
  • Biosensor Technology
  • Molecular Diagnostics

Background:

  • Anaplasma marginale is a significant cattle pathogen.
  • Accurate detection of anti-Anaplasma antibodies is crucial for disease management and vaccination efficacy.
  • Existing diagnostic methods may have limitations in sensitivity or accessibility.

Purpose of the Study:

  • To develop and validate an optical immunosensor for detecting antibodies against Anaplasma marginale.
  • To utilize recombinant major surface protein 5 (MSP5) for antibody detection.
  • To assess the performance of the immunosensor in cattle sera from enzootic and tick-free regions.

Main Methods:

  • Development of an optical immunosensor using recombinant MSP5 immobilized on controlled pore glass (CPG) beads.
  • Detection of anti-MSP5 antibodies in cattle serum samples.
  • Quantification based on Cy5 fluorescence measurement of a secondary antibody (anti-bovine IgG).

Main Results:

  • The immunosensor demonstrated a detection range of 1.2 µg/ml to 48 µg/ml for antibodies.
  • Achieved a sensitivity of 93% and a specificity of 70% in detecting anti-Anaplasma antibodies.
  • Successfully quantified antibodies in serum samples from naturally or experimentally infected cattle.

Conclusions:

  • The developed optical immunosensor is suitable for quantifying antibodies against Anaplasma marginale.
  • This technology can aid in diagnosing acute infections and evaluating vaccination responses in cattle.
  • The immunosensor offers a potential tool for epidemiological studies and herd health management.

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