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Updated: Jul 18, 2026

Assaying Protein Kinase Activity with Radiolabeled ATP
Published on: May 26, 2017
MAP kinases: differential activation following in vivo and ex vivo irradiation
Anirban Kumar Mitra1, Rakesh Kumar Singh, Malini Krishna
1Radiation Biology and Health Sciences Division, Bhabha Atomic Research Centre, Trombay, Mumbai, 400085, India.
Abstract:
Mitogen activated protein kinases (MAPK) play a critical role in controlling cell survival and repopulation following exposure to ionising radiation. Most investigations on these pathways have been done using cultured cells or by ex vivo treatments. The present study was carried out to determine whether the response of MAPKs in mouse lymphocytes differs following in vivo and ex vivo irradiation with 60Co gamma-rays. We observed that ex vivo treatment resulted in a very significant decrease in the activated p44/42 and p38 MAPK as compared to in vivo. However, stress activated protein kinase (SAPK) response showed no significant difference between in vivo and ex vivo treatments. These observations point towards the differences in response elicited when the treatment is given in vivo as compared to in vitro. Therefore the findings reported from in vitro or ex vivo treatments should be treated with caution especially if it has to be clinically applied.
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