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Efficient purification of transglutaminase from recombinant Streptomyces platensis at various scales

Shie-Jea Lin1, Yi-Fang Hsieh, Pei-Ming Wang

  • 1Food Industry Research and Development Institute, Hsinchu, Taiwan, ROC. lsj@firdi.org.tw

Biotechnology Letters
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PubMed

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Proteins are involved in several cellular processes and biochemical reactions. Analyzing a specific protein of interest requires it to be isolated from the other proteins in the cell. This is achieved by overexpressing the specific gene in a suitable host to produce large quantities of the target protein. A tag or label is recombined with the gene to produce a fusion protein containing the target protein and the tag. The tags on these fusion proteins can then be used for easy detection and...