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Related Experiment Videos

MEKC: an update focusing on practical aspects.

Manuel Silva1

  • 1Department of Analytical Chemistry, University of Cordoba, Cordoba, Spain. qa1sirom@uco.es

Electrophoresis
|December 1, 2006
PubMed
Summary

This review covers new methods and instruments for Micellar Electrokinetic Chromatography (MEKC). Advances in sample concentration, derivatization, and buffer composition enhance sensitivity and resolution for MEKC analysis.

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Area of Science:

  • Analytical Chemistry
  • Separation Science

Background:

  • Micellar Electrokinetic Chromatography (MEKC) is a powerful separation technique.
  • Continuous advancements are crucial for improving its analytical performance.

Purpose of the Study:

  • To review recent methodological and instrumental progress in MEKC.
  • To highlight techniques enhancing sensitivity and resolution.
  • To discuss the application of Mass Spectrometry (MS) and Laser-Induced Fluorescence (LIF) spectroscopy.

Main Methods:

  • On-line sample concentration techniques (sweeping, stacking).
  • In-capillary derivatization and flow-injection system coupling.
  • Modification of Background Electroendosmosis (BGE) composition (organic modifiers, ionic liquids, surfactants, mixed micelles, vesicles).
  • Use of coated capillaries.

Main Results:

  • Significant improvements in sensitivity achieved through on-line sample concentration and derivatization.
  • Enhanced resolution by optimizing BGE composition and employing coated capillaries.
  • Evaluation of MS and LIF spectroscopy for MEKC analysis, detailing their advantages and limitations.

Conclusions:

  • Recent advances have substantially improved MEKC sensitivity and resolution.
  • MS and LIF spectroscopy offer valuable detection capabilities for MEKC.
  • Future directions in MEKC methodology and instrumentation are promising.

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