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Using Multi-fluorinated Bile Acids and In Vivo Magnetic Resonance Imaging to Measure Bile Acid Transport
Published on: November 27, 2016
Isolation and determination of bile acids.
J Kandrac1, S Kevresan, J K Gu
1Faculty of Agriculture, Department of Chemistry, University of Novi Sad, Serbia.
European Journal of Drug Metabolism and Pharmacokinetics
|December 2, 2006
Summary
This review details methods for isolating and determining bile acids from animal sources. High-performance liquid chromatography is highlighted as the preferred technique for bile acid analysis.
Area of Science:
- Biochemistry
- Analytical Chemistry
Background:
- Bile acids are crucial biological molecules involved in digestion and metabolism.
- Accurate isolation and determination methods are essential for research and clinical applications.
Purpose of the Study:
- To provide a comprehensive review of bile acid isolation and determination techniques.
- To detail methods for separating and purifying bile acids from cattle and pig bile.
- To survey various analytical methods for quantifying bile acids in biological samples.
Main Methods:
- Detailed protocols for isolating and purifying bile acids from cattle and pig bile.
- Review of enzyme assays, radioimmunoassay, and enzyme immunoassay for bile acid determination.
- In-depth analysis of chromatographic techniques including thin-layer chromatography (TLC), gas chromatography (GC), and high-performance liquid chromatography (HPLC).
Main Results:
- Specific procedures for isolating cholic acid, deoxycholic acids, and hyodeoxycholic acid are described.
- Various analytical methods are evaluated for their efficacy in bile acid determination.
- High-performance liquid chromatography (HPLC) emerges as the most widely adopted method for bile acid separation and quantification.
Conclusions:
- A comprehensive overview of bile acid isolation and determination methods is presented.
- HPLC is identified as the current gold standard for bile acid analysis due to its efficiency and accuracy.
- The article serves as a valuable resource for researchers and clinicians working with bile acids.
