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Updated: Jul 18, 2026

Deep Proteome Profiling by Isobaric Labeling, Extensive Liquid Chromatography, Mass Spectrometry, and Software-assisted Quantification
Published on: November 15, 2017
Monolithic silica columns of various format in automated sample clean-up/multidimensional liquid chromatography/mass
Egidijus Machtejevas1, Sven Andrecht, Dieter Lubda
1Institut fuer Anorganische Chemie und Analytische Chemie, Johannes Gutenberg-Universitaet, Duesbergweg 10-14, D-55099 Mainz, Germany. egidijusmachtejevas@ahoo.com
Abstract:
The following particulate and monolithic silica columns were implemented in a fully automated and flexible multidimensional LC/MS system with integrated sample clean-up, to perform the analysis of endogeneous peptides from filtered urine and plasma samples: restricted access sulphonic acid strong cation-exchanger (RAM-SCX) for sample clean-up, RP 18 Chromolith guard columns as trap columns and 100 microm I.D. monolithic RP 18 fused silica capillary columns as last LC dimension. The results show sufficient overall system reproducibility and repeatability. Implementation of monolithic silica columns added an additional flexibility with respect to flow rate variation and adjustment due to the low column back pressures. Also, monolithic columns showed a lower clogging rate in long-term usage for biological samples as compared to particulate columns. The applied system set-up was tested to be useful for the routine peptide screening in search of disease biomarkers.
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