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A technique for isolation of bone marrow cells using hydroxyethyl starch (HES) sedimentation agent
A Montuoro1, L De Rosa, C Del Monte
1Divisione di Ematologia, Ospedale San Camillo, Rome, Italy.
Haematologica
|March 1, 1991
Summary
This study presents a manual method using hydroxyethyl starch (HES) to concentrate hemopoietic stem cells. This technique effectively isolates stem cells while minimizing red blood cell transfusion and progenitor cell loss, preventing delayed engraftment.
Area of Science:
- Hematology
- Cell Biology
- Biotechnology
Background:
- Hemopoietic stem cell (HSC) isolation is crucial for in vitro treatments and cryopreservation.
- Current methods involve significant cell manipulation and vary in stem cell fraction purity.
Purpose of the Study:
- To describe a manual method for concentrating hemopoietic stem cells using hydroxyethyl starch (HES).
- To evaluate the efficacy of HES in isolating mononuclear cells and removing unwanted components.
Main Methods:
- Bone marrow samples (n=54) were treated with hydroxyethyl starch (HES).
- A manual technique was employed to isolate the mononuclear cell population.
Main Results:
- HES facilitated the concentration of marrow stem cells into a small volume.
- Unwanted granulocytes, red blood cells, and donor isohemagglutinins were effectively removed.
- The method allowed minimal red blood cell transfusion without depleting progenitor cells.
Conclusions:
- The described manual HES method provides efficient hemopoietic stem cell concentration.
- This technique minimizes red blood cell contamination and preserves progenitor cell viability.
- It offers a viable approach to prevent delayed engraftment in stem cell transplantation.