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Related Experiment Video

Updated: Jul 18, 2026

The Automated Crystallography Pipelines at the EMBL HTX Facility in Grenoble
06:50

The Automated Crystallography Pipelines at the EMBL HTX Facility in Grenoble

Published on: June 5, 2021

The open-access high-throughput crystallization facility at EMBL Hamburg.

Jochen Mueller-Dieckmann1

  • 1EMBL Hamburg Outstation, c/o DESY, Notkestrasse 85, D-22603 Hamburg, Germany. jochenmd@embl-hamburg.de

Acta Crystallographica. Section D, Biological Crystallography
|December 2, 2006
PubMed
Summary

Europe's largest high-throughput crystallization facility is now open access, streamlining protein structure determination. This facility supports the entire crystallization workflow, from cocktail preparation to automated imaging, accelerating scientific discovery.

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Area of Science:

  • Structural Biology
  • Biochemistry
  • Materials Science

Background:

  • High-throughput crystallization is crucial for determining protein structures.
  • Access to advanced crystallization facilities can be limited for researchers.
  • Automated platforms enhance efficiency and reproducibility in crystallization experiments.

Purpose of the Study:

  • To report the establishment of Europe's largest open-access high-throughput crystallization facility.
  • To describe the comprehensive services offered, covering the entire crystallization workflow.
  • To detail the operational aspects and user engagement during the first year.

Main Methods:

  • Development of an integrated facility for crystallization cocktail preparation, experiment setup (hanging-drop vapor diffusion), and automated imaging.

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  • Implementation of an electronic booking system for user registration and parameter selection.
  • Provision of over 1000 initial crystallization conditions and 100+ stock solutions for customized screens.

    • Main Results:

    • The facility processed over 500 samples across 2985 crystallization plates from 43 internal and 40 external users in its first year.
    • Automated imaging captured 15 images per experiment over a six-month period.
    • A remote viewing system was implemented for internet-based experiment inspection.

    Conclusions:

    • The established facility significantly enhances research capabilities in structural biology by providing broad access to advanced high-throughput crystallization technologies.
    • The integrated workflow and user-friendly systems facilitate efficient protein structure determination for a diverse user community.
    • The facility's successful operation demonstrates a valuable resource for advancing scientific research in Europe.