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Published on: July 6, 2013
Intra- and intergenotypic variations among human cytomegalovirus gB genotypes
S Chantaraarphonkun1, P Bhattarakosol
1Inter-Department of Medical Microbiology, Graduate School, Chulalongkorn University, Bangkok, Thailand.
Objectives:
To study genotypic variations among human cytomegalovirus (HCMV) gB genotypes in clinical samples of Thai patients.
Methods:
gB genotyping of 31 HCMV-DNA-positive clinical samples were determined by PCR-RFLP, gene cloning and DNA sequencing methods.
Results:
Eight gB1, 7 gB2, 7 gB3, 6 gB untype (UT1 and UT2) and 3 mixed gB genotypes were first identified by PCR-RFLP. All 3 mixed gB genotype samples and 1 gB2 sample were cloned and confirmed gB genotype by PCR-RFLP. Altogether, 57 strains (27 unique types and 30 transformants) were further analyzed by DNA sequencing method. Discordant results between PCR-RFLP and DNA sequencing methods were demonstrated in 5 samples and 1 clone. The gB UT1 and UT2 were all classified as gB1 except 1 sample of UT1 (9D), suggesting a new variant. Each genotype had a similarity of more than 97%, whereas strains of different genotypes had 77.71-92.75% homology. The most divergent type was gB3.
Conclusions:
Intra- and intergenotypic variations among strains were demonstrated either in individual or distinct patients. Intragenotypic variation in a person occurs possibly due mainly to a point mutation mechanism rather than reinfection of a new gB genotype.
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