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Updated: Jul 18, 2026

Competitive Genomic Screens of Barcoded Yeast Libraries
Published on: August 11, 2011
Signature-tagged mutagenesis: barcoding mutants for genome-wide screens
Piotr Mazurkiewicz1, Christoph M Tang, Charles Boone
1Department of Infectious Diseases, Centre for Molecular Microbiology and Infection, Imperial College London, Armstrong Road, London SW7 2AZ, UK.
Abstract:
DNA signature tags (molecular barcodes) facilitate functional screens by identifying mutants in mixed populations that have a reduced or increased adaptation to a particular environment. Many innovative adaptations and refinements in the technology have been described since its original use with Salmonella; they have yielded a wealth of information on a broad range of biological processes--mainly in bacteria, but also in yeast and other fungi, viruses, parasites and, most recently, in mammalian cells. By combining whole-genome microarrays and comprehensive ordered libraries of mutants, high-throughput functional screens can now be achieved on a genomic scale.
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