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ADAM12-s in coelomic fluid and maternal serum in early pregnancy

George Makrydimas1, Alexandros Sotiriadis, Kevin Spencer

  • 1Department of Obstetrics and Gynaecology, Ioannina University Hospital, 45500 Ioannina, Greece.

Prenatal Diagnosis
|December 2, 2006
PubMed

Insights

Maternal serum ADAM12-s levels increase with gestation in early pregnancy. This placental protein was detected in maternal serum and coelomic fluid, but not amniotic fluid, supporting its syncytiotrophoblast origin.

Area of Science:

  • Reproductive biology
  • Maternal-fetal medicine
  • Biochemistry

Background:

  • ADAM12-s is a placental protein linked to adverse pregnancy outcomes like trisomy, pre-eclampsia, and fetal growth restriction.
  • Understanding ADAM12-s distribution in early pregnancy is crucial for potential diagnostic applications.

Purpose of the Study:

  • To investigate the distribution of ADAM12-s in maternal serum, amniotic fluid, and coelomic fluid during early pregnancy.
  • To compare ADAM12-s concentrations across these different biological compartments.

Main Methods:

  • Coelomic fluid, maternal serum, and amniotic fluid samples were collected from 13 singleton pregnancies (6.9-9.3 weeks gestation).
  • ADAM12-s concentrations were quantified using dissociation-enhanced lanthanide fluoro-immunoassay (DELFIA).

Main Results:

  • Median ADAM12-s concentration was 132.7 ng/mL in maternal serum and 10.5 ng/mL in coelomic fluid.
  • Detectable ADAM12-s levels were absent in most amniotic fluid samples (5/6).
  • Maternal serum ADAM12-s significantly correlated with gestational age (r=0.862, p<0.0001).

Conclusions:

  • The presence of ADAM12-s in maternal serum and coelomic fluid supports its origin from the syncytiotrophoblast.
  • ADAM12-s distribution patterns in early pregnancy warrant further investigation for clinical relevance.
Abstract

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