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Related Experiment Videos

Specific antibodies enhance alternative complement pathway activation by cuprophane.

F Maillet1, M D Kazatchkine

  • 1INSERM U 28, Hôpital Broussais, Paris, France.

Nephrology, Dialysis, Transplantation : Official Publication of the European Dialysis and Transplant Association - European Renal Association
|January 1, 1991
PubMed
Summary

Cuprophane activates the human alternative complement pathway, but activation levels vary individually. Anti-dextran antibodies influence this activation, suggesting a way to predict risks during hemodialysis.

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Area of Science:

  • Immunology
  • Biochemistry

Background:

  • Cuprophane is known to activate the human alternative complement pathway.
  • Individual variability in complement activation by cuprophane has been observed in vitro.

Purpose of the Study:

  • To investigate the factors influencing individual differences in alternative complement pathway activation by cuprophane.
  • To determine the role of anti-dextran antibodies in cuprophane-induced complement activation.

Main Methods:

  • Correlating cuprophane-induced alternative pathway activation with Sephadex-induced activation.
  • Measuring serum anti-dextran IgG antibody titers.
  • Assessing the effect of Sephadex or cuprophane adsorption on serum complement activation.

Main Results:

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  • Alternative pathway activation by cuprophane correlated with Sephadex activation, which is antibody-dependent.
  • Higher serum anti-dextran IgG antibody titers were associated with greater cuprophane-induced complement activation.
  • Adsorbing serum with Sephadex or cuprophane diminished subsequent complement activation by cuprophane.

Conclusions:

  • Anti-dextran antibodies play a significant role in the alternative complement pathway activation induced by cuprophane.
  • Measuring anti-dextran antibody levels may help identify patients at higher risk of complement activation during hemodialysis with cuprophane membranes.