Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Hematopoietic cells.

Malcolm A S Moore1, Jae-Hung Shieh, Gabsang Lee

  • 1Moore Laboratory, Cell Biology Program, Memorial Sloan-Kettering Cancer Center, New York, New York, USA.

Methods in Enzymology
|December 5, 2006
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Ectopic engraftment of nociceptive neurons derived from hPSCs for pain relief and joint homeostasis.

bioRxiv : the preprint server for biology·2025
Same author

Optogenetic activation of TGFβ signaling drives ligand-free chondrogenesis in hESC-derived MSCs.

Stem cells (Dayton, Ohio)·2025
Same author

Adenovirus E4ORF1 activates isoform-specific phosphatidylinositol 3-kinase signaling in human endothelial cells.

The Journal of biological chemistry·2025
Same author

A Rho GTPase-effector ensemble governs cell migration behavior.

Nature communications·2025
Same author

A non-canonical aryl hydrocarbon receptor pathway authorizes and safeguards clinical-scale expansion of functional human endothelial cells.

Nature cardiovascular research·2025
Same author

Transcriptional activation of regenerative hematopoiesis via microenvironmental sensing.

Nature immunology·2025
Same journal

Clinical Europium fluorescent based lectin assays for mucin O-glycomics.

Methods in enzymology·2026
Same journal

A dual-color FRET assay for detection and quantitative analysis of O-glycopeptidases.

Methods in enzymology·2026
Same journal

Evolutionary genetic approaches to analyze mucins.

Methods in enzymology·2026
Same journal

Ex vivo imaging and enzymatic analysis of intestinal mucus.

Methods in enzymology·2026
Same journal

Glyco-TRAPP: A real-time glycocalyx permeability assay for assessing transmembrane mucin barrier function in live and fixed tissues.

Methods in enzymology·2026
Same journal

Quantitative imaging approaches to capture structural and functional dynamics of colonic mucus in health and disease in situ.

Methods in enzymology·2026
See all related articles

Generating functional hematopoietic stem cells (HSC) from embryonic stem cells (ESC) remains challenging. Genetic manipulation shows promise for enhancing HSC engraftment, paving the way for clinical applications.

Area of Science:

  • Stem cell biology
  • Hematopoiesis
  • Developmental biology

Background:

  • Embryonic stem cells (ESC) can differentiate into hematopoietic lineages in vitro.
  • Current methods mimic early hematopoietic development but fail to produce engraftable hematopoietic stem cells (HSC).

Purpose of the Study:

  • To review current strategies for generating hematopoietic cells from ESC.
  • To identify obstacles and potential solutions for producing clinically relevant HSC from ESC.

Main Methods:

  • Formation of embryoid bodies (EB) from murine ESC (mESC) and human ESC (hESC).
  • Co-culture systems with bone marrow stromal cell lines.
  • Addition of specific growth factors and morphogens.
  • Selective culture systems for immune cell differentiation.

Related Experiment Videos

  • Genetic manipulation of mESC.

    • Main Results:

    • ESC differentiation generates primitive and definitive hematopoietic cells, including various mature blood cell types.
    • ESC differentiation largely recapitulates the yolk sac phase of hematopoiesis.
    • A major limitation is the inability to generate engraftable HSC.
    • Genetic upregulation of HOXB4 or STAT5 in mESC enhances hematopoietic engraftment.

    Conclusions:

    • ESC culture systems can generate diverse hematopoietic cells, but not yet clinically viable HSC.
    • Understanding the developmental timing of HSC emergence is crucial.
    • Genetic or epigenetic manipulation of key pathways may enable functional HSC generation from ESC for therapeutic use.